High-performance liquid chromatography-mass spectrometer is a powerful analytical detection tool that uses mass spectrometer as a detection method and integrates the high separation capability of high-performance liquid chromatography and the high sensitivity and selectivity of mass spectrometer. In recent years, with the maturity of soft ionization technologies such as electrospray and atmospheric pressure chemical ionization, their qualitative and quantitative analysis results have become more reliable and have become an indispensable analytical and detection tool in clinical pharmaceutical research.
1. Application in in vitro drug metabolite screening
High performance liquid chromatography mass spectrometry is the most suitable and effective analytical detection tool for the screening and identification of drug metabolites. Using high-performance liquid chromatography mass spectrometry, a wealth of information can be obtained from a wide variety of samples, which greatly improves the detection and identification capabilities of various drugs and their metabolites. Currently, most drug-like compounds are analyzed by electrospray ionization mass spectrometry, and compounds with less polarity are analyzed by atmospheric pressure chemical ionization mass spectrometry.
2. Application in the detection of drug-reactive metabolites
High performance liquid chromatography-mass spectrometry plays a leading role in the detection, identification and quantification of drug-reactive metabolites. In early drug discovery, HPLC-MS is used to rapidly screen and structure confirm reactive metabolites for lead optimization and candidate selection and development. Typically, the test substance is first incubated with liver microsomes in the presence of a capture agent, followed by detection and structure confirmation of the reactive metabolites captured by the capture agent using high-performance liquid chromatography-mass spectrometry. The results of these in vitro screening assays provide an indication of a given chemical type. and important information on potential metabolic activation of compounds of concern. High-performance liquid chromatography-mass spectrometry also plays an important role in the late stages of drug discovery and clinical development.
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